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61.
James R. Hagler Charles G. Jackson Thomas J. Henneberry Juli R. Gould 《Biocontrol Science and Technology》2002,12(6):661-675
In this study, we validate and apply techniques for marking and capturing small parasitoids of the silverleaf whitefly, Bemisia argentifolii Bellows & Perring [ = B. tabaci (Gennadius), strain B] for mark-release-recapture (MRR) studies. The marker is the purified protein, rabbit immunoglobulin G (IgG), which was applied externally by topical spray or internally by feeding. Marked parasitoids were then assayed using a sandwich enzyme-linked immunosorbent assay (ELISA) for the presence of the protein marker using an antibody specific to rabbit IgG. Virtually all of the externally marked Eretmocerus sp. (Ethiopia, M96076) (98.0%) contained enough rabbit IgG to be easily distinguished from unmarked parasitoids, regardless of the amount of protein applied or the post-marking interval. A field MRR study was then conducted to examine the dispersal characteristics of E. emiratus Zolnerowich & Rose. Parasitoids marked externally and internally with protein were released on three separate trial dates into the center of a cotton field bordered by cantaloupe and okra. Overall, a total of 1388, 637, and 397 marked and unmarked wasps were captured in suction traps during each trial, respectively with the majority of parasitoids captured between 0600 and 0800 h. Furthermore, even though we released an equal proportion of males to females, our traps consistently contained more males. Our results suggest that there are gender-specific differences in the dispersal behavior of E. emiratus . Almost 40% of the captured parasitoids collected during the three release trials were positively identified for the presence of the protein marker. The distribution of the marked parasitoids revealed two distinct patterns. First, almost all of the marked parasitoids recaptured in the cotton plot were in suction traps at or adjacent to the 相似文献
62.
Hernández-Ledesma Ana Laura Rodríguez-Méndez Adriana Jheny Gallardo-Vidal Lilia Susana García-Gasca Teresa Alatorre-Cruz Julia María García-Solís Pablo López Reyes Julián Solís-Saínz Juan Carlos 《Molecular biology reports》2020,47(12):9667-9676
Molecular Biology Reports - Although cognitive impairment (CI) is classically associated with aging, it has been proposed that neurological pathologies may increase the risk to suffer CI. Despite... 相似文献
63.
Nucleoid Condensation and Cell Division in Escherichia coli MX74T2 ts52 After Inhibition of Protein Synthesis 总被引:16,自引:16,他引:0
The reorganization of the bacterial nucleoid of an Escherichia coli mutant, MX74T2 ts52, was studied by electron microscopy after protein synthesis inhibition by using whole mounts of cell ghosts, ultrathin-sectioning, and freeze-etching. The bacterial nucleoid showed two morphological changes after chloramphenicol addition: deoxyribonucleic acid (DNA) localization and DNA condensation. DNA localization was observed 10 min after chloramphenicol addition; the DNA appeared as a compact, solid mass. DNA condensation was observed at 25 min; the nucleoid appeared as a cytoplasm-filled sphere, often opened at one end. Ribosomes were observed in the center. Giant nucleoids present in some mutant filaments showed fused, spherical nucleoids arranged linearly, suggesting that the tertiary structure of the nucleoid reflects the number of replicated genomes. Inhibitors which directly or indirectly blocked protein synthesis and caused DNA condensation were chloramphenicol, puromycin, amino acid starvation, rifampicin, or carbonyl cyanide m-chlorophenyl hydrazone. All inhibitors that caused cell division in the mutant also caused condensation, although some inhibitors caused condensation without cell division. Nucleoid condensation appears to be related to chromosome structure rather than to DNA segregation upon cell division. 相似文献
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65.
Ivonne Figueroa-González Gloria Moreno Julián Carrillo-Reyes Arturo Sánchez Guillermo Quijano Germán Buitrón 《Biotechnology letters》2018,40(3):569-575
Objectives
To assess the effect of one-step temperature increase, from 35 to 55 °C, on the methane production of a mesophilic granular sludge (MGS) treating wine vinasses and the effluent of a hydrogenogenic upflow anaerobic sludge blanket (UASB) reactor.Results
One-step temperature increase from mesophilic to thermophilic conditions improved methane production regardless of the substrate tested. The biomethane potentials obtained under thermophilic conditions were 1.8–2.9 times higher than those obtained under mesophilic conditions. The MGS also performed better than an acclimated thermophilic digestate, producing 2.2–2.5 times more methane than the digestate under thermophilic conditions. Increasing the temperature from 35 to 55 °C also improved the methane production rate of the MGS (up to 9.4 times faster) and reduced the lag time (up to 1.9 times). Although the temperature increase mediated a decrease in the size of the sludge granules, no negative effects on the performance of the MGS was observed under thermophilic conditions.Conclusions
More methane is obtained from real agroindustrial effluents at thermophilic conditions than under mesophilic conditions. One-step temperature increase (instead of progressive sequential increases) can be used to implement the thermophilic anaerobic digestion processes with MGS.66.
Rodrigo Megía-Palma Javier Martínez José J. Cuervo Josabel Belliure Octavio Jiménez-Robles Verónica Gomes Carlos Cabido Juli G. Pausas Patrick S. Fitze José Martín Santiago Merino 《International journal for parasitology》2018,48(9-10):709-718
Current and past parasite transmission may depend on the overlap of host distributions, potentially affecting parasite specificity and co-evolutionary processes. Nonetheless, parasite diversification may take place in sympatry when parasites are transmitted by vectors with low mobility. Here, we test the co-speciation hypothesis between lizard final hosts of the Family Lacertidae, and blood parasites of the genus Schellackia, which are potentially transmitted by haematophagous mites. The effects of current distributional overlap of host species on parasite specificity are also investigated. We sampled 27 localities on the Iberian Peninsula and three in northern Africa, and collected blood samples from 981 individual lizards of seven genera and 18 species. The overall prevalence of infection by parasites of the genus Schellackia was ~35%. We detected 16 Schellackia haplotypes of the 18S rRNA gene, revealing that the genus Schellackia is more diverse than previously thought. Phylogenetic analyses showed that Schellackia haplotypes grouped into two main monophyletic clades, the first including those detected in host species endemic to the Mediterranean region and the second those detected in host genera Acanthodactylus, Zootoca and Takydromus. All but one of the Schellackia haplotypes exhibited a high degree of host specificity at the generic level and 78.5% of them exclusively infected single host species. Some host species within the genera Podarcis (six species) and Iberolacerta (two species) were infected by three non-specific haplotypes of Schellackia, suggesting that host switching might have positively influenced past diversification of the genus. However, the results supported the idea that current host switching is rare because there existed a significant positive correlation between the number of exclusive parasite haplotypes and the number of host species with current sympatric distribution. This result, together with significant support for host–parasite molecular co-speciation, suggests that parasites of the genus Schellackia co-evolved with their lizard hosts. 相似文献
67.
Julián Vicente-Page Alfonso Pérez-Escudero Gonzalo G. de Polavieja 《Theoretical Ecology》2018,11(1):71-81
According to the classic results of Galton and Condorcet, as well as in modern decision-making models, accuracy in groups increases with group size. However, these studies do not consider the naturally occurring situation in which individuals dynamically re-evaluate their decision with a possible change of opinion. The dynamics of re-evaluation in groups are very different to individual re-evaluation because individuals influence the group and the group influences the individual. We find that individual accuracy in a group is higher when individuals re-evaluate because all members have more access to social information, while in single decisions, those deciding first have less. This improvement is smaller in large groups as in this case errors can cascade across the members of the group before re-evaluation can correct them. The net result is a maximal accuracy at a small group size. We also analyzed the case in which individuals are influenced only by a small number of the other individuals. In this case, cascading errors affect the interacting subgroups but are very unlikely to reach the whole group. This results in a local optimum at a small group size but also an optimum at a very large size. We thus suggest that re-evaluation dynamics can make small and very large groups optimal. Also, features that may be seen as limitations, like an influence from only a small number of individuals, may turn to be beneficial when considering local animal interactions, here filtering out cascading of errors in the group when reconsideration dynamics takes place. 相似文献
68.
Berta?Caballero-LópezEmail author José?M.?Blanco-Moreno Juli?Pujade-Villar Daniel?Ventura Josep?A.?Sánchez-Espigares F.?Xavier?Sans 《Journal of Insect Conservation》2016,20(3):467-476
Understanding ecosystem functioning in a farmland context by considering the variety of ecological strategies employed by arthropods is a core challenge in ecology and conservation science. We adopted a functional approach in an assessment of the relationship between three functional plant groups (grasses, broad-leaves and legumes) and the arthropod community in winter wheat fields in a Mediterranean dryland context. We sampled the arthropod community as thoroughly as possible with a combination of suction catching and flight-interception trapping. All specimens were identified to the appropriate taxonomic level (family, genus or species) and classified according to their form of feeding: chewing-herbivores, sucking-herbivores, flower-consumers, omnivores, saprovores, parasitoids or predators. We found, a richer plant community favoured a greater diversity of herbivores and, in turn, a richness of herbivores and saprovores enhanced the communities of their natural enemies, which supports the classical trophic structure hypothesis. Grass cover had a positive effect on sucking-herbivores, saprovores and their natural enemies and is probably due to grasses’ ability to provide, either directly or indirectly, alternative resources or simply by offering better environmental conditions. By including legumes in agroecosystems we can improve the conservation of beneficial arthropods like predators or parasitoids, and enhance the provision of ecosystem services such as natural pest control. 相似文献
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70.